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KMID : 0364819870250010073
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Korean Journal of Microbiology
1987 Volume.25 No. 1 p.73 ~ p.79
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Isolation of Kpn I restriction endonuclease from Klebsiella pneumonia
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Abstract
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1
A restriction endonuclease, Kpn I has been isolated from Klebsiella pneumonia. Cells were broken by sonication. After ultracentrifugation the supernatant containing Kpn I activity was further purified by Sepharose-6B gel filtration, DEAE-Cellulose, Heparin -Agarose, and Aminohexyl-Agarose column chromatography. Final enzyme preparation was essentially free of contaminating exonuclease and phosphatase, as judged by ligation-recut test. Total activity of the enzyme recovered from 10 grams of cells was 4.7 X 105 units.
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